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Literature summary for 3.5.1.44 extracted from

  • Qu, R.; Zhu, X.; Tian, M.; Liu, Y.; Yan, W.; Ye, J.; Gao, H.; Huang, J.
    Complete genome sequence and characterization of a protein-glutaminase producing strain, Chryseobacterium proteolyticum QSH1265 (2018), Front. Microbiol., 9, 1975 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene prgA, DNA and amino acid sequence determintion and analysis, genetic organization analysis Chryseobacterium proteolyticum

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the pre-pro-enzyme has a 21 amino acid signal peptide Chryseobacterium proteolyticum
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Organism

Organism UniProt Comment Textmining
Chryseobacterium proteolyticum Q9AQQ8 isolated from a soil sample collected in Songjiang, Shanghai, China
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Chryseobacterium proteolyticum QSH1265 Q9AQQ8 isolated from a soil sample collected in Songjiang, Shanghai, China
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is expressed as pre-pro-enzyme with a 21 amino acid signal peptide and a pro-sequence of 114 amino acids, which are cleaved off. The mature enzyme has 185 amino acids Chryseobacterium proteolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxybenzoxy-Gln-Gly + H2O
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Chryseobacterium proteolyticum ?
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?
carboxybenzoxy-Gln-Gly + H2O
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Chryseobacterium proteolyticum QSH1265 ?
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?
additional information the microbial enzyme specifically catalyzes deamidation of proteins without protein hydrolysis pretreatment and only reacts with glutamine residues in the side-chains of proteins or long peptides. Strain QSH1265 can produce acid from D-glucose, maltose, L-arabinose, sucrose, glycerol, and mannitol but not fructose, and it is also positive for indole production and urease activity Chryseobacterium proteolyticum ?
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?
additional information the microbial enzyme specifically catalyzes deamidation of proteins without protein hydrolysis pretreatment and only reacts with glutamine residues in the side-chains of proteins or long peptides. Strain QSH1265 can produce acid from D-glucose, maltose, L-arabinose, sucrose, glycerol, and mannitol but not fructose, and it is also positive for indole production and urease activity Chryseobacterium proteolyticum QSH1265 ?
-
?

Subunits

Subunits Comment Organism
More the enzyme is expressed as pre-pro-enzyme with a 21 amino acid signal peptide and a pro-sequence of 114 amino acids, the mature enzyme has 185 amino acids Chryseobacterium proteolyticum

Synonyms

Synonyms Comment Organism
prgA
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Chryseobacterium proteolyticum
protein-glutaminase
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Chryseobacterium proteolyticum